Yeah...I usually tend to run progressive brews so that the first pitch is not dry-hopped but I routinely run an IPA right on top of the yeast cake after I rack off a dry-hopped pale. Again, the amount and type of hops you used matter so you have to know what sort of beer you're running on the second pitch.
Also, if you do this, you should cool the wort very well - 60 degrees for an ale isn't too low - because it'll kick into gear immediately and you'll have a hell of a krausen before you know it. Be sure your fermenting vessel is large enough. There's enough yeast in a 1-gallon pitch for easily 5 to 10 gallons of wort, so whatever you pitch on the cake will go very quickly. Lower temp through out the fermentation is a good idea.
If you're continuing to do 1-gallon batches, just put the whole cake into a large jar, hops and all with enough clean water to dilute it well. Swirl it around, put it in the fridge and let it settle. Then the hops and protein break material will be on the bottom and the yeast will settle into a layer over that. You can rack out the water and spoon out the yeast, relatively free of hops. Probably a tablespoon full of good yeast trub will be plenty for a gallon of typical wort.