Hi, I know this thread is several years old, but the same issue exists now (the C. White Shaking option doesn't respond to changes in starter gravity). I see the explanation makes sense (this option is based on a very limited study that doesn't account for certain variables). There is another issue that I'm wondering if anyone can help with. The only options for aeration method on the calculator are Shaking, No Agitation, and Stir Plate. Do these all assume pure 02 aeration up front? I didn't think that was the case, but maybe it is. If the 'Shaking' option, for example, assumes that shaking (without 02 injection) is the only aeration method, then pure 02 up front with no agitation afterward I would think would produce much more growth than merely shaking (but not as much as continuous aeration with a stir plate).
If anyone has further info on this it would be greatly appreciated. In other words, I'm assuming that heavy pure 02 up front in the starter (with no agitation afterwards) would produce a significantly higher cell count than merely Shaking (but not as much as stir plate). If that's true it would be great to have some sense of where in between the result might fall, because there is a massive difference in cell count in the calculator between Shaking and Stir Plate. These differences are very large.