I was sure I could break down all my equipment and keep it clean since I have worked in a microbiology lab. Wrong. After 36 batches, I had a visible film in my clear tubing and a sure sign I was headed for an infection. Wort will surely grow a bio-film quicker and better than anything I've seen. Some have shared in these forums about using Star San for general sanitation use and the need to use a caustic like PBW for buildups. The PBW worked and I gave everything a soak, rinse, and a shot of Star San. What is the recommended period for PBW cleaning of brewing equipment?